Click-it edu alexa fluor imaging kit
WebInvitrogen™ Click-iT™ Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 594 dye. Designed to label and detect incorporated EdU and perform cell cycle analysis on … WebThis trial-size kit provides sufficient EdU to label up to 6–30 coverslips and enough Alexa Fluor™ 488, 594, and 647 azide to detect new DNA synthesis on 2–10 coverslips with each fluorophore. • Simple—works the …
Click-it edu alexa fluor imaging kit
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Webrabbit serum (A6455) and visualized with an Alexa Fluor® 488 goat anti-rabbit IgG antibody (A11034). Green fluorescence is also seen in the endoplasmic reticulum as it is formed … Webin PBS. Cells were saponin-permeabilized and EdU was detected with the Click-iT® EdU Alexa Fluor® 488 Flow Cytometry Kit (Invitrogen Cat. no. C35002). Phosphorylated …
WebClick-iT™ EdU Alexa Fluor® High-Throughput Imaging (HCS) Assay Kit 4 Before You Begin Materials Required but Not Provided Phosphate buffered saline (PBS, Invitrogen … WebMEFs were labeled with EdU (10 μM) using the Click -iT EdU Alexa Fluor 647 Imaging Kit (Thermo Fisher Scientific, C10340). Briefly, after overnight incubation with EdU, cells were fixed for 10 min in 3.7% …
WebIntroduction. チミジンの類縁体である EdU (5-ethynil-2'-deoxyuridine; 5-エチニル-2'-デオキシウリジン) は DNA に取り込まれるため、EdU 存在下でゲノム DNA を合成した (分裂増殖した) 細胞の核を EdU で標識することができる。. Materials. 培養液; Click-iT EdU Alexa Fluor Imaging Kit (Thermo, C10337) WebClick-iT® RNA Imaging Kits 5 1.2 To prepare a stock solution of the Alexa Fluor® azide: • Add 94 μL of DMSO to Alexa Fluor® 488 vial (Component B, Cat. no. C10329), and mix well by pipetting or vortexing. • Add 85 μL of DMSO to Alexa Fluor® 594 vial (Component B, Cat. no. C10330), and mix well by pipetting or vortexing.
WebThe black outlined histogram is the cells stained with CD3 mouse anti-Human mAb PE conjugate and Click-iT ® Plus EdU Alexa Fluor 647 picolyl azide. The gray outlined …
WebThe Click-iT EdU cell proliferation assay (Invitrogen) for detection of replicating cells is based on incorporation of EdU into newly synthesized DNA and its recognition by azide dyes via a copper mediated "click" reaction. In the protocol provided by Invitrogen, cells are fixed with paraformaldehyde and stained with 7-aminoactinomycin D (7-AAD ... ge predix investmentge premier washer dryerWebMar 9, 2015 · Click-iT® EdU Imaging Kits . Table 1. Contents and storage information. Material C10337. C10338 C10339: C10340 Concentration: Storage* EdU (Component … geprc tinygo racing fpv whoop rtf kitWebClick-iT® EdU Flow Cytometry Assay Kits *50 tests* 2 Figure 1 Fluorescence signal from Pacific Blue™, Alexa Fluor® 488, and Alexa Fluor® 647 Click-iT® EdU Flow Cytometry Assay Kits. Jurkat (human T-cell leukemia) cells were treated with 10 µM EdU for 2 hours and detected according to the recommended staining protocol. ge pre-lit winterberry 5\u0027 branch treeWebProduct overview. EdU Assay / EdU Staining Proliferation Kit (iFluor 488) ab219801 provides a sensitive and robust method to detect and quantify cell proliferation in live mammalian cells using flow cytometry or fluorescence microscopy. The iFluor 488 dye (Ex/Em: 491/520 nm) has spectral properties almost identical to those of FITC and ... christie leatiotaWebThermo Fisher Scientific - US christie learning zone log inWebClick-iT™ EdU Alexa Fluor® High-Throughput Imaging (HCS) Assay Kit 5 2–6˚C. When stored as directed, this 1X solution is stable for 6 months. 1.5 To make a 10X stock solution of the Click-iT™ EdU buffer additive (Component F), add 2 mL deionized water to the vial and mix until fully dissolved. After use, store any remaining stock christie leary attorney