WebFeb 17, 2024 · Add liquid nitrogen to the tissue and roughly grind the sample into the mortar and pestle. After the tissue becomes a powder, add 500 μL of CTAB extraction buffer and beta-mercaptoethanol, grind it … WebCTAB (also called hexadecyltrimethylammonium bromide) is a cationic detergent that facilitates the separation of polysaccharides during purification while additives, such as …
CTAB Protocol For Isolating DNA From Plants: OHM
WebMar 14, 2024 · The chloroform extraction should be executed quickly within ~ 5 min. 10. When pipetting nuclei solution from organic extraction, using cut (large orifice) 5-ml tip. 11. Volume ratio of the 10% CTAB versus the supernatant should be 1:10. 12. During the last organic extraction, take caution not to disturb the interphase. WebJan 1, 2024 · The optimized CTAB DNA extraction method using forest elephant dung samples yields DNA concentrations ranging from 6.2 to 1716.9 ng/μL, far greater than the one derived from the method of Benbouza et al. [25] that ranged from -49.4 to 16.3 ng/μL. In addition to yield a high DNA concentration, our optimized method presents a relatively … 髄膜炎菌 ワクチン接種
A Rapid Publishing Journal - ResearchGate
WebJun 4, 2024 · Lastly, I tried regular CTAB extraction. both 260/280 and 260/230 are good; 1.86 and 1.9 respectively. However, the band size of the extracted DNA is little lower than the kit extracted. Web500 μl of CTAB buffer. 2. Transfer CTAB/plant extract mixture to a microfuge tube. 3. Incubate the CTAB/plant extract mixture for about 15 min at 55° C in a recirculating water bath. 4. After incubation, spin the CTAB/plant extract mixture at 12000 g for 5 min to spin down cell debris. Transfer the supernatant to clean microfuge tubes. 5. WebJul 26, 2024 · Herein, optimization of zeolite NaA/NaX synthesis conditions in order to obtain the final product with high surface area and pore volume was investigated. An optimal synthesis condition was 5 days aging time and crystallization time of 9 h with the co-addition of cetyltrimethylammonium bromide (CTAB) and heptane. All those optimal synthesis … 髄膜炎 ワクチン 種類