Trizon reagent cwbio
WebJan 24, 2024 · Total RNA extraction was performed using the TRIzol reagent (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions, and RNA samples were purified using the RNeasy mini kit (Qiagen, Germantown, MD, USA). ... CWBIO; Beijing, China). Blots were detected using the ECL-enhanced chemiluminescence reagent (CWBio, … WebMar 1, 2024 · Total RNA was extracted from CytPM-COS and mock treatment N. tobacum K326 using TRIzon Reagent (CWBIO, Beijing, China). Thereafter, cDNA was synthesized …
Trizon reagent cwbio
Did you know?
WebNov 27, 2024 · For each sample, approximately 2,000 synchronized young adult worms were collected and resuspended in TRIzon Reagent(CW0580, CWBIO). Total RNA was isolated by chloroform extraction, followed by isopropanol precipitation. RNA libraries were prepared for sequencing using standard Illumina protocols. PolyA RNA was selected. Library strategy: … WebAug 25, 2016 · RNA extraction was carried out using TRIzon Reagent (CWBio, China) following the manufacturer’s instructions. Possibly contaminated genomic DNA was removed by DNase (Invitrogen, USA) treatment. The OD 260 :OD 280 ratio of total RNA was detected with Nanodrop 2000 (Thermo Scientific Inc., USA).
WebTRIzon Reagent TRIzon 总RNA提取试剂 TRIzon是广谱型总RNA提取试剂。实验操作快速方便，颜色鲜明 . 品牌：CWBIO. 询价. 石家庄乐为科技有限公司 ... WebNov 26, 2024 · Total RNA was extracted from the samples using TRIzon reagent RNA kit (CWbio). RNA (1 μg) was reverse transcribed with TransScriptR one-step gDNA Removal and cDNA Synthesis SuperMix (Transgen Biotech Co., Ltd.). The …
WebJan 19, 2024 · Many nitrate transporters, transcription factors, and protein kinases participate in the regulation of nitrate signaling. Here, we identified a gene encoding the chrysanthemum calcineurin B-like... Web1a.植物组织：取新鲜植物组织在液氮中充分研磨或将植物组织剪碎后直接在TRIzon 中迅速研磨，每30-50 mg组织加入1 ml TRIzon，混匀。 注意：样品体积一般不要超过TRIzon体积 …
WebTRIzon是广谱型总RNA提取试剂。. 实验操作快速方便，颜色鲜明，便于分层。. 本试剂适用范围广泛，可以从动物组织、植物材料、各种微生物及培养细胞等样品中提取总RNA。. …
WebJan 27, 2024 · Total RNA was extracted from Chrysanthemum leaves using a TRIzon Reagent (CW0580S, Cwbio, China). The HiFiScript gDNA Removal RT MasterMix (CW 2024S, Cwbio, China) was used for reverse transcription to synthesize cDNA. The nucleotide sequence of CIPK8 was obtained from the Chrysanthemum Genome Database … do deposits go through on weekendsWebInvitrogen TRIzol products are referenced in thousands of journal publications, reflecting the trust that molecular biologists have placed in TRIzol Reagent to deliver high-quality, intact … exworks exgoWebNov 22, 2024 · Total RNA was extracted with Trizon Reagent (Cwbio, China). The first-strand cDNA was prepared using the HiFiScript cDNA Synthesis kit (Cwbio, China) according to … do deposits from hotels get refundedWebAug 7, 2024 · For every 1 ml of 0.64 mg/ml CS solution, 71 µ l of 1 mg/ml filtered sodium tripolyphosphate in 0.1% v/v acetic acid/ddH 2 O containing pDNA (2% w/w of final NP mass yield) was added slowly to the solution under 1000-rpm stirring using a Scilogex OS20-Pro overhead stirrer (Scilogex, Rocky Hill, CT) to achieve ionic gelation. do depressed people feel sorry for themselvesWebApr 14, 2024 · Every 50 μL PCR assay reaction mixture consisted of 25 μL of 2× PCR premix Taq polymerase (CWBio, Beijing, China), 1 μL of the DNA template, 0.5 μL of each primer, and 21 μL of water. PCR amplification consisted of an initial denaturation step at 94°C for 2 min, followed by 30 cycles of denaturation at 94°C for 1 min, annealing at 66°C ... ex works chineseWebSep 19, 2024 · Total RNA of maize roots (~ 2000 ng) was extracted by using the TRIzon Reagent Kit (CWBIO). 1 μg total RNA was reverse transcribed for quantitative PCR by using a HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) Kit (Vazyme). Quantitative PCR analyses were conducted by using a SYBR Green Fast qPCR Mix (ABclonal). do depressed people lack serotoninWebMar 1, 2024 · After cell transfection, total RNA was extracted using Trizon reagent (CW0580, CWBIO, China) and reversely transcribed to cDNA using HiFiScript cDNA synthesis kit (CW2141, CWBIO, China) according to the manufacturer’s instructions. do depressed people have messy rooms